This was probably one of the most complex and technologically advanced procedures currently being performed in our country in preclinical and clinical research,” is how Prof. Kovacs says of the kidney autotransplant surgery. Ph. n. med. Maciej Kosieradzki of Warsaw Medical University. The team led by him transplanted the organ, which was left uncirculated for about 30 minutes and then stored for 12 hours at a temperature only slightly below normal body temperature. Without using blood. NanOX is a comprehensive and proprietary system of devices and innovative fluids for extracorporeal storage, as well as organ regeneration for transplantation. It consists of NanOX 4K perfusion fluid, the NanOX Recovery Box universal perfusion device, which monitors vital organ functions, and a proprietary perfusion protocol. The usefulness of the solution has been confirmed by a series of experiments, the last of which, in 2023, resulted in successful perfusion of a kidney, which lasted about 6 days. They prepared the research team, led by Prof. Maciej Kosieradzki, MD, of Warsaw Medical University, president of the Polish Transplantation Society, for the most important test, the autotransplantation operation, which took place in July 2024. – We were the first to successfully perform a kidney autotransplantation procedure using synthetic hemoglobin-depleted preservative fluid under subnormal conditions. In practice, this was a successful preclinical trial, but the patient could not yet be human, so we performed it in a domestic pig – This is what Prof. Kosieradzki says about the experiment. Kosieradzki, who combines his work on the project with his duties as head of the General and Transplant Clinic at the Infant Jesus Clinical Hospital.
Course of the experiment
The experiment consisted of taking one kidney from an animal after a 30-minute cardiac arrest, perfusing it with NanOX fluid and using the NanOX Recovery Box device under extracorporeal conditions in subnormothermia, i.e. at 25 degrees Celsius, for 12 hours. This technology, combined with the unique NanOX 4K fluid, which uses nanoparticles to deliver oxygen to the organ and take away carbon dioxide, makes it possible to provide the organ with the necessary substrates for metabolism, oxygenation and nutrition. In practice, this means that the kidney has not only retained its suitability for transplantation throughout this time, but also regeneration processes have been taking place in it. In the next step, after the researchers removed the second kidney, they implanted the animal with the organ, which had previously undergone extracorporeal perfusion. Then, for the next two weeks, intensive observation was carried out, monitoring the health of the “patient” and the function of the transplanted kidney. – Already on the eighth day after the procedure, the animal became fully functional, as did the transplanted kidney, which was confirmed by the results of biochemical tests,” recalled Dr. n. biol. Eng. Jaroslaw Wolinski of the Polish Academy of Sciences.
Further prospects
Successful autotransplantation is an important step toward commercialization of the NanOX system, according to Piotr Mierzejewski. – The results of our experiment open up new possibilities in the field of transplantation. We have proven that our system not only allows organs to be stored much longer, but also makes it possible to regenerate them and, who knows, in the future, to treat them as well – evaluates the vice president of NanoGroup S.A. Read more about our project and autotransplantation: TVP3 Warsaw (24.07.2024), TVP1 Teleexpress, Warsaw University of Technology, MAMBIZNES.PL, BUSINESS PULSE, COWZDROWIU.PL, BANKIER.PL, RYNEKZDROWIA.PL, CEO.COM.PL, HEJTO.PL, MEDICALPRESS.PL, GOSPODARKA.SOS.PL, PULS MEDYCYNY, ISB HEALTH, PAP BUSINESS, BIOTECHNOLOGIA.PL, MZDROWIE.PL, STREFA INWESTORÓW, LABNEWS, INFOSTREFA, GPW.
